Reagents
PacBio RS reagent kits are available in several packaging options emphasizing the granularity and flexibility of our system. You can sequence DNA samples in an individual SMRT™ Cell or across multiple SMRT Cells according to your experiment design and data needs. This customizable run capability provides for cost efficiencies in both large and small sequencing projects.
The PacBio RS front-end preparation and on-instrument sequencing workflow is supported by three reagent kits.
Front End Preparation
The DNA template preparation and DNA polymerase binding steps may be performed manually at the benchtop or by an automated liquid-handling system.
1. DNA Template Preparation Reagent Kits
Isolated DNA samples are converted into primer-annealed SMRTbell™ library format.
2. DNA Polymerase Binding Reagent Kits
Prepared DNA samples, in SMRTbell library format, are bound to the DNA polymerase in preparation for sequencing.
DNA Sequencing
The DNA sequencing step is performed on the PacBio RS. Sequencing reagent kits supply all materials needed for easy instrument loading.
3. DNA Sequencing Reagent Kit
Supports automated, on-instrument DNA sequencing. Available in standard or strobe protocols for longer DNA template libraries. Used in conjunction with SMRT Cell 8Pacs.
Standard Sequencing Kits
- DNA Template Prep Kit 8 and 24 Reaction
- DNA/Polymerase Binding Kit 8 and 24 Reaction
- DNA Sequencing Kit 8 Reaction
The DNA Template Prep Kit contains reagent supplies to perform DNA template library preparation of primer-annealed SMRTbellTM library. Reagent quantities support 8 or 24 library preparations
The DNA/Polymerase Binding Kit contains reagent supplies to bind prepared DNA template library to the DNA polymerase. Reagent quantities support 8 or 24 binding reactions.
The DNA Sequencing Kit contains reagent supplies for single molecule real time DNA sequencing on the PacBio RS instrument to be used in conjuction with SMRTTM Cell 8Pac(s). Reagent quantities support 8 sequencing reactions.
Strobe Sequencing Kits
The Strobe Template Prep Kit contains reagent supplies to perform DNA template library preparation of longer templates. Reagent quantities support 8 library preparations.
The Strobe Binding Kit contains reagent supplies to bind longer DNA template libraries to the DNA polymerase. Reagent quantities support 8 binding reactions.
The Strobe Sequencing Kit contains reagent supplies for single molecule real-time strobe sequencing on the PacBio RS instrument to be used in conjunction with SMRT Cell 8Pac(s). Reagent quantities support 8 sequencing reactions.
The PacBio RS family of proprietary consumables provides all the components you need for customizable single molecule, real-time (SMRT™) DNA sequencing.
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