Sequencing with the PacBio RS system based on our single molecule, real-time (SMRT™) technology offers the following key benefits:
Single molecule, real-time analysis
SMRT technology harnesses the power of the DNA polymerase to enable single molecule, real-time sequencing. The ability to resolve single molecules in real time allows our system to observe structural and cell type variation not accessible with other technologies. Unlike existing sequencing platforms, minimal amounts of reagent and sample preparation are required and there are no time-consuming flushing, scanning and washing steps. In addition, our platform does not require the routine PCR amplification needed by most second generation sequencing systems thereby avoiding systematic amplification bias.
Longer readlengths
The PacBio RS is designed to produce readlengths greater than 1,000 base pairs on average with instances of over 10,000 base pairs which facilitates mapping and assembly. Longer readlengths require the sequencing of fewer overlapping segments, referred to as coverage, to efficiently assemble the underlying genomic structure. Most second generation technologies require higher coverage to compensate for short readlengths. However, even with high coverage, short readlengths are difficult to assemble, especially in highly repetitive areas of the genome. In addition, long readlengths are an important factor in enabling a comprehensive view of the genome, as they can reveal multiple types of genetic variation, such as large-scale rearrangements as observed in cancer. We believe that the long readlengths produced by the PacBio RS will allow insights into biology that are not possible with existing technologies.
Faster time to result
With the PacBio RS, sample preparation to sequencing results can take less than one day. A typical sequencing run can require as little as 30 minutes of instrument time. Sequence data is produced in minutes rather than days, potentially enabling important applications including infectious disease monitoring and molecular pathology.
